活体分子显微镜观测

A novel type of biomedical imaging, made possible by new advances in microscopy from scientists at Harvard University, is so fast and sensitive it can capture "video" of blood cells squeezing through（挤过，勉强通过） capillaries¹（毛细血管） . Researchers led by Harvard's Brian G. Saar, Christian² W. Freudiger, and X. Sunney Xie describe the work this week in the journal Science.

The new technique, based on stimulated³ Raman scattering⁴ (SRS), makes a complementary partner to MRI, widely used to capture static images of organs, tumors, and other large structures. For the first time, SRS microscopy makes possible label-free chemical movies, with streaming footage at the subcellular（亚细胞的） level, catching⁵ video of proteins, lipids, and water within cells.

"When we started this project 11 years ago, we never imagined we'd have an amazing result like this," says Xie, professor of chemistry and chemical biology at Harvard. "It took MRI more than 30 years to reach patients, but we're already looking forward with great anticipation⁶ to applications of SRS microscopy in hospitals. It's now clear that stimulated Raman scattering will play an important role in the future of biological imaging and medical diagnostics."

Xie says SRS microscopy could aid, and speed, surgery to remove tumors and other lesions（损害） . Surgeons must now send excised⁷ samples for histological（组织学的） analysis -- a process that takes about 20 minutes -- while a patient waits on the operating table. SRS microscopy provides equivalent insights through real-time scanning.

Xie's team has already used SRS microscopy to track migration⁸ of medications in skin, shedding new light on the absorption of topical（局部的，论题的） drugs. In conjunction with（连同，共同） endoscopy（内窥镜检查） , the technique can also view three-dimensional sections of tissue, layer by layer.

"Previous SRS microscopy captured only about one image per minute, far too slow for use in live animals or humans," Xie says. "We were able to speed the collection of data by more than three orders of magnitude, attaining⁹ video-rate imaging."

Because SRS microscopy works by detecting the intrinsic（本质的，固有的） vibrations¹⁰ in chemical bonds between atoms, it doesn't require intrusive¹¹ fluorescent¹² labeling. An optical technique, it compliments MRI, whose depth of penetration¹³ is better suited to imaging organs and other large objects deep within the body.

The current work greatly improves detection of signals -- backscattered（反向散射） by tissues in the body -- by rearranging photodetectors to surround a small aperture¹⁴（孔，穴） through which a beam of light is directed at the tissue being examined. Using this approach, the scientists were able to collect and analyze¹⁵ almost 30 percent of the laser light directed at a biological sample, a more than 30-fold increase over previous SRS microscopy.

Scientists currently use a variety of techniques to view biomolecules, but most have significant limitations that are sidestepped by SRS microscopy. Labeling with green fluorescent protein (GFP) provides sharp images, but the bulky protein can perturb¹⁷（扰乱） delicate biological pathways, overwhelming smaller biomolecules. Conventional infrared¹⁸ (IR) microscopy has low spatial¹⁹ resolution and requires desiccated（干的） samples, while spontaneous Raman microscopy requires high laser power and long integration²⁰ times, limiting use in live specimens²¹. Coherent anti-Stokes Raman scattering (CARS) microscopy, pioneered by Xie's own group, lacks the contrast to image most molecules¹⁶ beyond lipids.