科学家创造出拟人化丙肝小鼠模型

Scientists at Rockefeller University and The Scripps Research Institute have developed the first genetically¹ humanized mouse model for hepatitis C（丙型肝炎） , an achievement that will enable researchers to test molecules³ that block entry of the hepatitis C virus into cells as well as potential vaccine⁴ candidates. The finding is reported in the June 9 issue of the journal Nature. While the hepatitis C virus can infect chimpanzees and humans, scientists have been unable to study the progression of the virus' life cycle or possible treatments in small animal models. The new mouse model is the first to be developed with a fully⁵ functioning immune system.

"Our genetically humanized mouse model for hepatitis C will allow us to gain deeper insights in the biology of this important pathogen（病原体） ," says senior author Alexander Ploss, a research assistant professor at Rockefeller. "This robust⁶ small animal model also has the potential to serve a critical role in testing and prioritizing drug and vaccine candidates. Results from these tests can potentially guide more expensive pre-clinical and clinical studies in higher order organisms, including humans."

The development of this mouse model is the culmination⁷ of several years of research by scientists in the laboratory of Charles M. Rice and other research groups. In 2006, Rice and his colleagues were the first to successfully create a strain of hepatitis C in the laboratory, which can efficiently⁸ be grown in the laboratory, and is also infectious in animals. More recently, Rice, Ploss and their colleagues discovered that hepatitis C virus infection requires previously⁹ identified CD81 and scavenger¹⁰ receptor type B class I, as well as two tight junction¹¹ molecules, claudin 1 and occludin（遮光蛋白） . The Rockefeller researchers showed that human CD81 and occludin were required for hepatitis C virus to enter mouse cells.

In the new study, the Rockefeller researchers and colleagues at The Scripps Research Institute tested whether introducing some of these previously identified human genes¹² into mice would allow them to infect the animals with the hepatitis C virus. The researchers compared two groups of mice: one group expressed two genes, CD81 and occludin, while mice in the second group were normal. They found that expression of human CD81 and human occludin in the mouse liver rendered the animals susceptible¹³ to HCV infection. Ploss and his colleagues also developed a novel reporter system, which allowed them to sensitively detect HCV infection in living animals.

"We have established a precedent¹⁴（在前的） for applying mouse genetics to dissect¹⁵ viral entry and validate¹⁶ the role of scavenger receptor type B class 1, a molecule² that is being considered as a novel antiviral drug target, for HCV uptake in a living animal," says Charles M. Rice, Maurice R. and Corinne P. Greenberg Professor and head of the Laboratory of Virology and Infectious Disease at Rockefeller. Rice also is executive and scientific director of the Center for the Study of Hepatitis C, an interdisciplinary center established jointly¹⁷ by The Rockefeller University, NewYork-Presbyterian Hospital and Weill Cornell Medical College.

Worldwide at least 130 million people are chronically¹⁸ infected with HCV, which poses a risk of severe liver injury and liver cancer. Current treatments are only partially¹⁹ effective and have considerable side effects, and a vaccine against hepatitis C does not exist.

"The global HCV epidemic²⁰ mandates²¹ the development of more effective therapeutics including a vaccine," says Ploss. "This mouse model is a first step toward a platform that effectively serves this purpose."