延时技术模拟卵巢肿瘤生长情况

Scientists at Harvard University have created a laboratory model using time-lapse（延时的） video microscopic¹ technology that allows observation of early stages of ovarian cancer metastasis. "We were able to observe key molecular² mechanisms⁴ that are necessary for the force-dependent processes associated with metastasis（转移，新陈代谢） ," said Joan Brugge, Ph.D., professor and chair of cell biology at Harvard University.

These findings are published in Cancer Discovery, the newest journal of the American Association for Cancer Research. According to Brugge, who served as program chairperson for the AACR 102nd Annual Meeting 2011, ovarian cancer cells spread throughout the peritoneum（腹膜） by attaching to the outer cell layer of organs in this area and then clearing away this layer of cells and embedding⁵ themselves on the organ, where they then proliferate⁶（激增，扩散） and expand.

"The reason these tumors are so morbid⁸（病态的） is that the metastatic tumors grow large enough to interfere⁹ with the function of the organs in the peritoneum," she said.

By using the time-lapse video microscopic technique, Brugge and colleagues were able to visualize¹⁰ the detailed¹¹ sequence of events associated with insertion of tumor⁷ cells into peritoneal monolayers in cell culture, and then determine that the mechanism³ involves tumor cells' use of force via 5 integrin（整合素） , talin I and muscle myosin II.

"Theoretically, by targeting these molecules¹², it may be possible to prevent the formation of new metastatic tumors," said Brugge.