细菌致病新机理被发现

Scientists have identified a new mechanism¹ of bacterial² pathogenesis（发病原理） . The results of the research project, partly funded by the Academy of Finland, have been published in the journal Proceedings³ of the National Academy of Sciences of the United States of America (PNAS). Bacteria that cause chronic⁴ infections have an amazing but yet poorly known ability to subvert⁵（颠覆，推翻） immune response, live and produce offspring, enter and wake up from a dormant⁶（休眠的） phase to cause, in some instances, deadly complications.

Bartonella bacteria cause chronic infections in mammals (incl. humans), and are typically transmitted to new hosts mainly by arthropod vectors such as fleas⁷, lice and ticks, but also via direct tissue trauma⁸ (e.g. cat scratches).

One very notable feature of these bacteria is their ability to cause vasoproliferative tumours⁹ that resemble Kaposi's sarcoma in patients suffering from immunodeficiency (e.g. AIDS, aggressive cancer treatments, organ transplantation). If left untreated, these foci of inflammation maintain a chronic infection and contribute to transmitting bacteria to new hosts.

In his research, biologist Arto Pulliainen (University of Turku) has demonstrated that Bartonella henselae injects a protein called BepA into vascular¹⁰ endothelial cells and that this protein manipulates cAMP-mediated cell signalling using a previously¹¹ unknown mechanism.

BepA directly binds¹² the host cell adenylyl cyclase, which is an enzyme¹³ responsible for the production of cAMP. However, the binding¹⁴ of BepA to the adenylyl（腺苷） cyclase does not activate¹⁵ cAMP production per se, but the adenylyl cyclase rather becomes more sensitive to its natural activator¹⁶, stimulatory¹⁷ G-protein (Gαs). The cellular¹⁸ concentration of cAMP increases and prevents the death of the host cell. BepA significantly prolongs the lifespan of the host cell and partly contributes to the formation of vasoproliferative tumours.

Several bacterial species are known to manipulate host cell functions via cAMP-mediated cell signalling. The symptoms are typically very strong and may even be deadly. The best-known example is Vibrio cholerae and its cholera¹⁹ toxin²⁰, which modifies Gαs into a permanently²¹ adenylyl cyclase-stimulating form. BepA, in turn, manipulates host cell signalling in a subtle sophisticated manner, which is ideal for chronic persistence²² of Bartonella henselae in the infected vascular（血管的） endothelium（内皮） .

The research has been carried out at the Universities of Basel and Turku.