Researchers at Washington University School of Medicine in St. Louis have learned why changes in a single gene¹, ROP18, contribute substantially（实质上，大体上） to dangerous forms of the parasite² Toxoplasma gondii（弓形虫） . The answer has likely moved science a step closer to new ways to beat Toxoplasma and many other parasites³. In a study published in Cell Host & Microbe, scientists show that the ROP18 protein disables host cell proteins that would otherwise pop a protective bubble the parasite makes for itself. The parasite puts the bubble on like a spacesuit by forming a membrane⁴ around itself when it enters host cells. This protects it from the hostile environment inside the cell, which would otherwise kill it.

"If we can find therapies that block ROP18 and other parasite proteins like it, that could give the host the upper hand（优势，占上风） in the battle against infection," says first author Sarah Fentress, a graduate student in the laboratory of L. David Sibley, PhD, professor of molecular⁵ microbiology.

Infection with Toxoplasma, or toxoplasmosis（弓形体病） , is most familiar to the general public from the recommendation that pregnant women avoid changing cat litter. Cats are commonly infected with the parasite, as are some livestock⁶ and wildlife.

"The exact role of ROP18 and related proteins in human disease remains⁷ to be studied," says Sibley. "But mice are natural hosts of Toxoplasma, so studies in laboratory mice are relevant to the spread of infection."

Epidemiologists estimate that as many as one in every four humans is infected with Toxoplasma. Infections typically cause serious disease only in patients with weakened immune systems. In some rare cases, though, infection in patients with healthy immune systems leads to serious eye or central nervous system disease, or congenital defects（先天性缺陷） or death in the fetuses⁸ of pregnant women.

In the new study, Fentress showed that the ROP18 protein binds⁹ to a class of host proteins known as immunity-related GTPases. Tests in cell cultures and animal models showed that this binding¹⁰ leads to a reaction that disables the GTPases, which normally would rupture（破裂） the parasite's protective membrane.

"With one exception, humans don't have the same family of immunity-related GTPases," Fentress notes. "But we do have a similar group of immune recognition proteins called guanylate-binding proteins, and we are currently testing to see if ROP18 deactivates¹² these proteins in human cells in a similar manner."

The findings could be applicable to other parasites and pathogens. Toxoplasmosis belongs to a family of parasites that includes the parasite Plasmodium, which causes malaria¹³. All surround themselves with protective membranes¹⁴ when they enter host cells.

"Plasmodium doesn't make ROP18, but it does secrete¹⁵ related proteins called FIKK," says Fentress. "It's possible they also act to thwart¹⁶（挫败，反对） host defense¹⁷ mechanisms¹⁸ like GTPases and guanylate（鸟甘酸） -binding proteins."