The International and American Associations for Dental Research have released in its Journal of Dental Research a study that investigated bone fluoride（氟化物） levels in individuals with osteosarcoma（骨肉瘤） , which is a rare, primary malignant¹ bone tumor² that is more prevalent in males. Since there has been controversy³ as to whether there is an association between fluoride and risk for osteosarcoma, the purpose of this study, titled "An Assessment⁴ of Bone Fluoride and Osteosarcoma," was to determine if bone fluoride levels were higher in individuals with osteosarcoma. No significant association between bone fluoride levels and osteosarcoma risk was detected in this case-control study, based on controls with other tumor diagnoses.

In the case-control study, by lead researcher Chester Douglass of Harvard University, patients were identified by physicians in the orthopedic（整形外科的） departments from nine hospitals across the U.S. between 1993 and 2000. In this report, the study sample included incident cases of primary osteosarcoma and a control group of patients with newly-diagnosed malignant bone tumors. Specimens⁵ of tumor-adjacent bone and iliac crest⁶ bone were analyzed⁷ for fluoride content. The study was approved by the Institutional Review Boards of the respective hospitals, Harvard Medical School and the Medical College of Georgia.

Logistic regression of the incident cases of osteosarcoma (N=137) and tumor controls (N=51), adjusting for age and sex and potential confounders of osteosarcoma, was used to estimate odds⁸ ratios (OR) and 95% confidence intervals⁹ (CI). There was no significant difference in bone fluoride levels between cases and controls. The OR adjusted for age, gender¹⁰, a history of broken bones was 1.33 (95% CI: 0.56-3.15).

"The controversy over whether there is an association between fluoride and risk for osteosarcoma has existed since an inconclusive animal study 20 years ago," said IADR Vice-president Helen Whelton. "Numerous human descriptive and case-control studies have attempted to address the controversy, but this study of using actual bone fluoride concentrations as a direct indicator¹¹ of fluoride exposure represents our best science to date and shows no association between fluoride in bone and osteosarcoma risk."