Researchers have shown for the first time in an animal that is more closely related to humans that it is possible to make new bone from stem-cell-like induced pluripotent（多能的） stem cells (iPSCs) made from an individual animal's own skin cells. The study in monkeys reported in the Cell Press journal Cell Reports on May 15th also shows that there is some risk that those iPSCs could seed tumors, but that unfortunate outcome appears to be less likely than studies in immune-compromised mice would suggest. "We have been able to design an animal model for testing of pluripotent stem cell therapies using the rhesus macaque（恒河猴）, a small monkey that is readily available and has been validated² as being closely related physiologically³ to humans," said Cynthia Dunbar of the National Heart, Lung, and Blood Institute. "We have used this model to demonstrate that tumor¹ formation of a type called a 'teratoma' from undifferentiated autologous（自体同源的） iPSCs does occur; however, tumor formation is very slow and requires large numbers of iPSCs given under very hospitable⁴ conditions. We have also shown that new bone can be produced from autologous iPSCs, as a model for their possible clinical application."

 

Autologous refers to the fact that the iPSCs capable of producing any tissue type -- in this case bone -- were derived⁵ from the very individual that later received them. That means that use of these cells in tissue repair would not require long-term or possibly toxic⁶ immune suppression drugs to prevent rejection⁷.

 

The researchers first used a standard recipe to reprogram skin cells taken from rhesus macaques. They then coaxed⁸ those cells to form first pluripotent stem cells and then cells that have the potential to act more specifically as bone progenitors⁹. Those progenitor¹⁰ cells were then seeded onto ceramic¹¹（陶瓷的） scaffolds that are already in use by reconstructive surgeons attempting to fill in or rebuild bone. And, it worked; the monkeys grew new bone.

 

Importantly, the researchers report that no teratoma structures developed in monkeys that had received the bone "stem cells." In other experiments, undifferentiated iPSCs did form teratomas（畸胎瘤） in a dose-dependent manner.

 

The researchers say that therapies based on this approach could be particularly beneficial for people with large congenital bone defects or other traumatic injuries. Although bone replacement¹² is an unlikely "first in human" use for stem cell therapies given that the condition it treats is not life threatening, the findings in a primate¹³ are an essential step on the path toward regenerative clinical medicine.

 

"A large animal preclinical model for the development of pluripotent or other high-risk/high-reward generative cell therapies is absolutely required to address issues of tissue integration¹⁴ or homing, risk of tumor formation, and immunogenicity," Dunbar said. "The testing of human-derived cells in vitro or in profoundly immunodeficient（免疫缺陷的） mice simply cannot model these crucial preclinical safety and efficiency issues."

 

The NIH team is now working with collaborators on differentiation¹⁵ of the macaque iPSCs into liver, heart, and white blood cells for eventual¹⁶ clinical trials in hepatitis C, heart failure, and chronic¹⁷ granulomatous（肉芽肿的） disease, respectively.

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