基因筛选有助致病菌的甄别
文章来源:未知 文章作者:meng 发布时间:2009-11-27 01:30 字体: [ ]  进入论坛
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Whitehead researchers have developed a new type of genetic2 screen for human cells to pinpoint3 specific genes4 and proteins used by pathogens(病原体), according to their paper in Science. In most human cell cultures genes are present in two copies: one inherited from the father and one from the mother. Gene1 inactivation5(钝化作用) by mutation6 is therefore inefficient7 because when one copy is inactivated8, the second copy usually remains9 active and takes over.

In yeast10(酵母), researchers have it easier: they use yeast cells in which all genes are present in only one copy (haploid单倍体 yeast). Now Carette and co-workers have used a similar approach and used a human cell line, in which nearly all human chromosomes11 are present in a single copy.

In this rare cell line, Carette and co-workers generated mutations in almost all human genes and used this collection to screen for the host genes used by pathogens. By exposing those cells to influenza12(流感) or to various bacterial13 toxins15(毒素), the authors isolated16 mutants that were resistant17 to them. Carette then identified the mutated genes in the surviving cells, which code for a transporter molecule18 and an enzyme19(酶) that the influenza virus hijacks20 to take over cells.

Working with Carla Guimaraes from Whitehead Member Hidde Ploegh's lab, Carette subjected knockout cells to several bacterial toxins to identify resistant cells and therefore the genes responsible.

The experiments identified a previously21 uncharacterized gene as essential for intoxication22 by diphtheria toxin14(白喉毒素) and exotoxin(外毒素) A toxicity23, and a cell surface protein needed for cytolethal distending24 toxin toxicity.

"We were surprised by the clarity of the results," says Jan Carette, a postdoctoral(博士后的) researcher in the Brummelkamp lab and first author on the Science article. "They allowed us to identify new genes and proteins involved in infectious processes that have been studied for decades, like diphtheria and the flu. In addition we found the first human genes essential for host-pathogen interactions where few details are known, as is the case for cytolethal distending toxin secreted25 by certain strains of E. coli(大肠杆菌). This could be important for rapidly responding to newly emerging pathogens or to study pathogen biology that has been difficult to study experimentally."

Brummelkamp sees the work as only the beginning.

"Having knockout cells for almost all human genes in our freezer opens up a wealth of biological questions that we can look at," he says. "In addition to many aspects of cell biology that can be studied, knockout screens could also be used to unravel26 molecular27 networks that are exploited by a battery of different viruses and bacteria."



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1 gene WgKxx     
n.遗传因子,基因
参考例句:
  • A single gene may have many effects.单一基因可能具有很多种效应。
  • The targeting of gene therapy has been paid close attention.其中基因治疗的靶向性是值得密切关注的问题之一。
2 genetic PgIxp     
adj.遗传的,遗传学的
参考例句:
  • It's very difficult to treat genetic diseases.遗传性疾病治疗起来很困难。
  • Each daughter cell can receive a full complement of the genetic information.每个子细胞可以收到遗传信息的一个完全补偿物。
3 pinpoint xNExL     
vt.准确地确定;用针标出…的精确位置
参考例句:
  • It is difficult to pinpoint when water problems of the modern age began.很难准确地指出,现代用水的问题是什么时候出现的。
  • I could pinpoint his precise location on a map.我能在地图上指明他的准确位置。
4 genes 01914f8eac35d7e14afa065217edd8c0     
n.基因( gene的名词复数 )
参考例句:
  • You have good genes from your parents, so you should live a long time. 你从父母那儿获得优良的基因,所以能够活得很长。 来自《简明英汉词典》
  • Differences will help to reveal the functions of the genes. 它们间的差异将会帮助我们揭开基因多种功能。 来自英汉非文学 - 生命科学 - 生物技术的世纪
5 inactivation 18ae5d115854e3d37d23b02949a1e431     
n.灭活,失活,钝化(作用);减化
参考例句:
  • This inactivation may be accomplished in a variety of ways. 这种失去活性作用能以多种方式来实现。 来自辞典例句
  • Long sonication periods can lead to some inactivation. 长时间的声波作用可导致某种程度的失活。 来自辞典例句
6 mutation t1PyM     
n.变化,变异,转变
参考例句:
  • People who have this mutation need less sleep than others.有这种突变的人需要的睡眠比其他人少。
  • So far the discussion has centered entirely around mutation in the strict sense.到目前为止,严格来讲,讨论完全集中于围绕突变问题上。
7 inefficient c76xm     
adj.效率低的,无效的
参考例句:
  • The inefficient operation cost the firm a lot of money.低效率的运作使该公司损失了许多钱。
  • Their communication systems are inefficient in the extreme.他们的通讯系统效率非常差。
8 inactivated 9301af139e2f8eb6dae70f855b1f1216     
v.使不活泼,阻止活动( inactivate的过去式和过去分词 )
参考例句:
  • Molluscicides are quickly inactivated by sunlight and adsorption to mud organic matter. 灭螺剂因阳光作用、泥土及有机物质的吸收会很快失效。 来自辞典例句
  • Viruses were inactivated by BPL and the toxicity measured again. BPL对病毒进行灭活,测定残存毒力。 来自互联网
9 remains 1kMzTy     
n.剩余物,残留物;遗体,遗迹
参考例句:
  • He ate the remains of food hungrily.他狼吞虎咽地吃剩余的食物。
  • The remains of the meal were fed to the dog.残羹剩饭喂狗了。
10 yeast 7VIzu     
n.酵母;酵母片;泡沫;v.发酵;起泡沫
参考例句:
  • Yeast can be used in making beer and bread.酵母可用于酿啤酒和发面包。
  • The yeast began to work.酵母开始发酵。
11 chromosomes 11783d79c0016b60332bbf1856b3f77d     
n.染色体( chromosome的名词复数 )
参考例句:
  • Chromosomes also determine the sex of animals. 染色体也决定动物的性别。 来自《简明英汉词典》
  • Each of four chromosomes divide longitudinally. 四种染色体的每一种都沿着纵向分裂。 来自《现代汉英综合大词典》
12 influenza J4NyD     
n.流行性感冒,流感
参考例句:
  • They took steps to prevent the spread of influenza.他们采取措施
  • Influenza is an infectious disease.流感是一种传染病。
13 bacterial dy5z8q     
a.细菌的
参考例句:
  • Bacterial reproduction is accelerated in weightless space. 在失重的空间,细菌繁殖加快了。
  • Brain lesions can be caused by bacterial infections. 大脑损伤可能由细菌感染引起。
14 toxin hn5wb     
n.毒素,毒质
参考例句:
  • Experts have linked this condition to a build-up of toxins in the body.专家已把这一病症与体内毒素的积累联系起来。
  • Tests showed increased levels of toxin in shellfish.检验表明水生有壳动物的毒素水平提高了。
15 toxins 18c3f40d432ba8dc33bad8fb82873ea8     
n.毒素( toxin的名词复数 )
参考例句:
  • The seas have been used as a receptacle for a range of industrial toxins. 海洋成了各种有毒工业废料的大容器。
  • Most toxins are naturally excreted from the body. 大部分毒素被自然排出体外。 来自《简明英汉词典》
16 isolated bqmzTd     
adj.与世隔绝的
参考例句:
  • His bad behaviour was just an isolated incident. 他的不良行为只是个别事件。
  • Patients with the disease should be isolated. 这种病的患者应予以隔离。
17 resistant 7Wvxh     
adj.(to)抵抗的,有抵抗力的
参考例句:
  • Many pests are resistant to the insecticide.许多害虫对这种杀虫剂有抵抗力。
  • They imposed their government by force on the resistant population.他们以武力把自己的统治强加在持反抗态度的人民头上。
18 molecule Y6Tzn     
n.分子,克分子
参考例句:
  • A molecule of water is made up of two atoms of hygrogen and one atom of oxygen.一个水分子是由P妈?f婘??妈?成的。
  • This gives us the structural formula of the molecule.这种方式给出了分子的结构式。
19 enzyme cPozF     
n.酵素,酶
参考例句:
  • Above a certain temperature,the enzyme molecule will become unfolded.超过一定温度,酶分子将会展开。
  • An enzyme that dissolves the fibrin of blood clots.能溶解血凝块中的纤维的酶。
20 hijacks 678bf5894e05ba78937af53c72ee0477     
劫持( hijack的名词复数 ); 绑架; 拦路抢劫; 操纵(会议等,以推销自己的意图)
参考例句:
  • Just as life infiltrates matter mercilessly and then hijacks it forever, cultural life hijacks biology. 正如生命无情地浸入物质并永远地将其劫持,文化生命也劫持了生物界。
21 previously bkzzzC     
adv.以前,先前(地)
参考例句:
  • The bicycle tyre blew out at a previously damaged point.自行车胎在以前损坏过的地方又爆开了。
  • Let me digress for a moment and explain what had happened previously.让我岔开一会儿,解释原先发生了什么。
22 intoxication qq7zL8     
n.wild excitement;drunkenness;poisoning
参考例句:
  • He began to drink, drank himself to intoxication, till he slept obliterated. 他一直喝,喝到他快要迷糊地睡着了。
  • Predator: Intoxication-Damage over time effect will now stack with other allies. Predator:Intoxication,持续性伤害的效果将会与队友相加。
23 toxicity InYwc     
n.毒性,毒力
参考例句:
  • The hoarse grunt or squeal is characteristic of toxicity.嘶哑的哼声和叫声是中毒的特征。
  • Dieldrin is related to aldrin,and its toxicity to earthworms is similar.狄氏剂与艾氏剂有关,对蚯蚓的毒性是相似的。
24 distending 3706b981cfee70b45ea0cc450c70a8a4     
v.(使)膨胀,肿胀( distend的现在分词 )
参考例句:
  • Carrie looked at him a moment, her eyes distending. 嘉莉瞪大两眼,看了他一会儿。 来自英汉文学 - 嘉莉妹妹
25 secreted a4714b3ddc8420a17efed0cdc6ce32bb     
v.(尤指动物或植物器官)分泌( secrete的过去式和过去分词 );隐匿,隐藏
参考例句:
  • Insulin is secreted by the pancreas. 胰岛素是胰腺分泌的。
  • He secreted his winnings in a drawer. 他把赢来的钱藏在抽届里。 来自《简明英汉词典》
26 unravel Ajzwo     
v.弄清楚(秘密);拆开,解开,松开
参考例句:
  • He was good with his hands and could unravel a knot or untangle yarn that others wouldn't even attempt.他的手很灵巧,其他人甚至都不敢尝试的一些难解的绳结或缠在一起的纱线,他都能解开。
  • This is the attitude that led him to unravel a mystery that long puzzled Chinese historians.正是这种态度使他解决了长期以来使中国历史学家们大惑不解的谜。
27 molecular mE9xh     
adj.分子的;克分子的
参考例句:
  • The research will provide direct insight into molecular mechanisms.这项研究将使人能够直接地了解分子的机理。
  • For the pressure to become zero, molecular bombardment must cease.当压强趋近于零时,分子的碰撞就停止了。
TAG标签: gene toxin yeast pathogen
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