Leafcutter ant queens can live for twenty years, fertilizing¹ millions of eggs with sperm² stored after a single day of sexual activity. Danish researchers who have studied ants at the Smithsonian Tropical Research Institute in Panama since 1992 discovered that in both ant and bee species in which queens have multiple mates, a male's seminal³ fluid（精液） favors the survival of its own sperm over the other males' sperm. However, once sperm has been stored, leafcutter ant queens neutralize⁴（中和，抵消） male-male sperm competition with glandular⁵（腺的） secretions⁶（分泌物） in their sperm-storage organ.

"Two things appear to be going on here," explains Jacobus Boomsma, professor at the University of Copenhagen and Research Associate at STRI. "Right after mating there is competition between sperm from different males. Sperm is expendable（消耗品） . Later, sperm becomes very precious to the female who will continue to use it for many years to fertilize⁷ her own eggs, producing the millions of workers it takes to maintain her colony."

With post-doctoral researchers Susanne den⁸ Boer in Copenhagen and Boris Baer at the University of Western Australia, professor Boomsma studied sperm competition in sister species of ants and bees that mate singly—each queen with just one male—or multiply—with several males.

Their results, published this week in the prestigious⁹（有名望的） journal, Science, show that the ability of a male's seminal fluid to harm the sperm of other males only occurs in species that mate multiply, and that their own seminal fluid does not protect sperm against these antagonistic¹⁰（敌对的，反对的） effects.

"Females belonging to many species—from vertebrates（脊椎动物） to insects-- have multiple male partners. Seminal products evolve rapidly, probably in response to the intense male-male competition that continues even after courtship（求爱，求婚） and mating have taken place," said William Eberhard, Smithsonian staff scientist. "This study continues the STRI tradition of looking at post-copulatory（交配的） selection in a very biodiverse range of organisms, following in the footsteps of people like Bob Silberglied, who asked why butterflies and moths¹¹（飞蛾） have two kinds of sperm in the 1970's."

Similar sperm competition systems appear to have evolved independently in ants and in bees. Researchers now aim to discover how genes¹² that control sperm recognition in bees and ants may differ, thus continuing to elucidate¹³（说明，阐明） the details of a process key to reproduction and evolution.