Leafcutter ant queens can live for twenty years, fertilizing1 millions of eggs with sperm2 stored after a single day of sexual activity. Danish researchers who have studied ants at the Smithsonian Tropical Research Institute in Panama since 1992 discovered that in both ant and bee species in which queens have multiple mates, a male's seminal3 fluid（精液） favors the survival of its own sperm over the other males' sperm. However, once sperm has been stored, leafcutter ant queens neutralize4（中和，抵消） male-male sperm competition with glandular5（腺的） secretions6（分泌物） in their sperm-storage organ.
"Two things appear to be going on here," explains Jacobus Boomsma, professor at the University of Copenhagen and Research Associate at STRI. "Right after mating there is competition between sperm from different males. Sperm is expendable（消耗品） . Later, sperm becomes very precious to the female who will continue to use it for many years to fertilize7 her own eggs, producing the millions of workers it takes to maintain her colony."
With post-doctoral researchers Susanne den8 Boer in Copenhagen and Boris Baer at the University of Western Australia, professor Boomsma studied sperm competition in sister species of ants and bees that mate singly—each queen with just one male—or multiply—with several males.
Their results, published this week in the prestigious9（有名望的） journal, Science, show that the ability of a male's seminal fluid to harm the sperm of other males only occurs in species that mate multiply, and that their own seminal fluid does not protect sperm against these antagonistic10（敌对的，反对的） effects.
"Females belonging to many species—from vertebrates（脊椎动物） to insects-- have multiple male partners. Seminal products evolve rapidly, probably in response to the intense male-male competition that continues even after courtship（求爱，求婚） and mating have taken place," said William Eberhard, Smithsonian staff scientist. "This study continues the STRI tradition of looking at post-copulatory（交配的） selection in a very biodiverse range of organisms, following in the footsteps of people like Bob Silberglied, who asked why butterflies and moths11（飞蛾） have two kinds of sperm in the 1970's."
Similar sperm competition systems appear to have evolved independently in ants and in bees. Researchers now aim to discover how genes12 that control sperm recognition in bees and ants may differ, thus continuing to elucidate13（说明，阐明） the details of a process key to reproduction and evolution.