A research team led by Cary Pirone from the Department of Biological Sciences at Florida International University has identified bilirubin（胆红素） in the popular Bird of Paradise（天堂鸟） plant. The breakthrough study, published in the September 2010 issue of the American Society for Horticultural（园艺的） Science's journal HortScience, provides new insights into color production in this iconic tropical plant. Previously¹ thought to be an "animal-only" pigment²（色素，颜料） , bilirubin is best known as the yellowish hue³（色彩，色度） associated with bruises⁴（擦伤，青肿） and jaundice（黄疸，偏见） sufferers. In 2009 the FIU researchers found bilirubin in the arils of Strelitzia（鹤望兰） nicolai, the white Bird of Paradise tree. The incredible discovery—that bilirubin exists in both plants and animals—put Pirone's research on the scientific map. The current study expands the original research and reveals new insights into the presence of animal pigment in flowers. Advisor⁵ David Lee credits Pirone for her persistence⁶ and scientific acumen⁷. "Cary has made a remarkable⁸ discovery", he noted⁹, adding that it was Pirone's persistence and curiosity that persuaded colleagues that she was on the right track.

Strelitzia reginae Aiton, the Bird of Paradise plant, is known for its vibrant¹⁰ orange and blue inflorescences（花，开花） . Native to South Africa, it is widely cultivated in warm temperate¹¹ and tropical regions. Aside from（除……以外） the widely recognized shape of its flower, which resembles the head of a bird, Strelitzia reginae is also admired for its brilliant floral（花的，植物的） coloration. In contrast to its showy flowers, the fruit of the Bird of Paradise is pale and partially¹² obscured by the bract（苞，苞片） during development. When it matures, however, the capsule breaks open to reveal intensely colored orange arillate（有假种皮的，有子衣的） seeds. Remarkably¹³, the distinct aril color can remain unchanged for decades after the plant dies.

Using high-performance liquid chromatography (HPLC) and HPLC/electrospray ionization–tandem mass spectrometry, the research team discovered bilirubin to be the primary aril pigment of Strelitzia reginae and found low concentrations of bilirubin in the plant's sepals. In mature aril tissue, bilirubin was present as granular（颗粒的） bodies irregularly distributed throughout the cell. In mature sepal（萼片） tissue, the researchers observed elongate¹⁴（伸长的，延长的） structures that were previously identified as containing carotenoids（类胡萝卜素） .

"This research is the first discovery of bilirubin in a flower; it verifies the presence of bilirubin in a plant species other than Strelitzia nicolai. With further research on the function, distribution, and synthesis of bilirubin in plants, the information may be useful for practical applications such as the manipulation of color through breeding and genetics", the researchers concluded.

The findings will likely have broad appeal among flower lovers, observed Lee. "When you discover something this significant about something this familiar (the Bird of Paradise flower), the story has power".