A virus not known to cause disease kills triple-negative breast cancer cells and killed tumors grown from these cells in mice, according to Penn State College of Medicine researchers. Understanding how the virus kills cancer may lead to new treatments for breast cancer. Adeno-associated virus type 2 (AAV2) infects humans but is not known to cause sickness. In prior studies, the researchers tested the virus on a variety of breast cancers that represent degrees of aggressiveness and on human papillomavirus-positive cervical（子宫颈的） cancer cells. The virus initiated² apoptosis（细胞死亡） -- natural cell death -- in cancer cells without affecting healthy cells.

 

"Treatment of breast cancer remains³ difficult because there are multiple signaling pathways that promote tumor¹ growth and develop resistance to treatment," said Craig Meyers, Ph.D., Distinguished⁴ Professor of Microbiology and Immunology.

 

Signaling pathways involve molecules⁶ in a cell that control cell functions -- such as cell division -- by cooperation. For example, the first molecule⁵ in the process receives a signal to begin. It then tells another molecule to work, and so on.

 

Treatment of breast cancer differs by patient due to differences in tumors. Some tumors contain protein receptors that are activated⁷ by the hormones⁹ estrogen or progesterone. Others respond to another protein called human epidermal¹⁰（表皮的） growth factor receptor 2, or HER2. Each of these is treated differently.

 

A triple-negative breast cancer does not have any of these protein receptors and is typically aggressive.

 

"There is an urgent and ongoing¹¹ need for the development of novel therapies which efficiently¹² target triple-negative breast cancers," Meyers said.

 

In the current study, the researchers tested AAV2 on a cell-line representative of triple-negative breast cancer. The researchers report their results in Cancer Biology & Therapy.

 

The AAV2 killed 100 percent of the cells in the laboratory by activating¹³ proteins called caspases, which are essential for the cell's natural death. In addition, consistent with past studies, AAV2-infected cancer cells produced more Ki-67, an immunity¹⁴ system activating protein and c-Myc, a protein that helps both to increase cell growth and induce apoptosis. The cancer cell growth slowed by day 17 and all cells were dead by day 21. AAV2 mediated¹⁵ cell killing¹⁶ of multiple breast cancer cell lines representing both low and high grades of cancer and targeted the cancer cells independent of hormone⁸ or growth factor classification.

 

The researchers then injected AAV2 into human breast cancer cell line-derived tumors in mice without functioning immune systems. Mice that received AAV2 outlived the untreated mice and did not show signs of being sick, unlike the untreated mice. Tumor sizes decreased in the treated mice, areas of cell death were visible and all AAV2 treated mice survived through the study, a direct contrast to the untreated mice.

 

"These results are significant, since tumor necrosis -- or death -- in response to therapy is also used as the measure of an effective chemotherapeutic（化学疗法的）," Meyers said.

 

Future studies should look at the use of AAV2 body-wide in mice, which would better model what happens in humans, according to Meyers.

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