Whether you're baking bread or building an organism, the key to success is consistently adding ingredients in the correct order and in the right amounts, according to a new genetic² study by University of Michigan researchers. Using the baker's yeast³ Saccharomyces cerevisiae, Patricia Wittkopp and her colleagues developed a novel way to disentangle the effects of random⁴ genetic mutations and natural selection on the evolution of gene¹ expression. Their findings are scheduled for online publication in the journal Nature on March 16.

 

"These results tell us that the effects of mutations available for natural selection to act on can play a large role in how an organism evolves. The approach we developed allows us to test what those influences are and to better understand how evolution works," said Wittkopp, an associate professor in the U-M Department of Ecology and Evolutionary⁶ Biology and in the Department of Molecular⁷, Cellular⁸ and Developmental Biology.

 

The first authors of the Nature paper are EEB graduate student Brian Metzger and former MCDB graduate student David Yuan.

 

In their study, the U-M researchers used the TDH3 gene, which helps baker's yeast cells convert sugar into energy used for growth. To understand how different genetic mutations impact TDH3 expression, they systematically⁹ changed each of the DNA¹⁰ base pairs in a region of the gene known as the promoter, which controls the amount of the gene that is expressed.

 

They created all of the different possible genetic mutations in this region and measured the change in gene expression caused by each mutation⁵.

 

"Surprisingly, we found that most mutations didn't change the average expression of TDH3 but instead changed how variable TDH3 expression was among genetically¹¹ identical cells," Wittkopp said.

 

"It would be as if you changed the recipe for a loaf of bread in a way that didn't change the average quality of the bread but instead caused individual loaves to be unpredictably good or bad," Metzger said.

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