Amphibian¹ populations living close to agricultural fields have become more resistant² to a common insecticide and are actually resistant to multiple common insecticides, according to two recent studies conducted at the University of Pittsburgh. Amphibian populations living close to agricultural fields have become more resistant to a common insecticide and are actually resistant to multiple common insecticides. In a study published today in Evolutionary³ Applications, the Pitt researchers demonstrate, for the first time, that tadpoles⁴（蝌蚪） from populations close to farm fields are more resistant to chlorpyrifos（毒死蜱） -- one of the most commonly applied⁵ insecticides in the world, often sold as "Dursban" or "Lorsban." In addition, a related study published in February shows that tadpoles resistant to chlorpyrifos are also resistant to other insecticides.

 

"While we've made a lot of progress in understanding the ecological⁶ consequences to animals that are unintentionally exposed to insecticides, the evolutionary consequences are poorly understood," said study principal investigator⁷ Rick Relyea, Pitt professor of biological sciences and director of the University's Pymatuning Laboratory of Ecology. "Our study is the first to explore how amphibian populations might evolve to be resistant to insecticides when they live in places that have been sprayed for many years."

 

The Pitt researchers used newly hatched tadpoles collected from nine populations of wood frogs living at different distances from agricultural fields. They tested the frogs' resistance when exposed to chlorpyrifos, which is used against insects, and Roundup Original MAX®, which is a common herbicide used against weeds.

 

Relyea and his Pitt collaborators exposed the tadpoles from each of the nine populations to environments containing either no pesticides⁸, chlorpyrifos, or Roundup®. After 48 hours, they measured how well the populations survived.

 

"Wood frogs living close to agricultural land were more likely to have been exposed to pesticides for many generations compared to those living far from agriculture; the latter frog populations likely experienced little or no exposure to pesticides," said Rickey Cothran, the lead author of the study and a postdoctoral researcher in Relyea's lab. "Although populations differed in their resistance to Roundup®, populations closer to fields were not more resistant to the herbicide."Wood frogs living close to agricultural land were more likely to have been exposed to pesticides for many generations compared to those living far from agriculture.

 

"Because chlorpyrifos kills in a way that is similar to many other insecticides, higher resistance may have been favored each time any insecticide was sprayed," said Pitt alumnus Jenise Brown (A&S '09), a coauthor of the study and a former undergraduate researcher in Relyea's lab. "In contrast, herbicides have a variety of ways that they kill organisms, which may make it harder for animals to be resistant when exposed to different herbicides over many years."

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