In work published this week in Nature: Scientific Reports, a team of researchers from the University of Notre Dame¹'s Eck Institute for Global Health, led by Associate Professor Giles Duffield and Assistant Professor Zain Syed of the Department of Biological Sciences, revealed that the major malaria² vector in Africa, the Anopheles gambiae mosquito, is able to smell major human host odorants（有气味的东西） better at night. The study reports an integrative approach to examine the mosquito's ability to smell across the 24-hour day and involved proteomic（蛋白质组学）, sensory³ physiological⁴, and behavioral techniques. The researchers examined the role for a major chemosensory family of mosquito proteins, odorant-binding proteins (OBPs), in the daily regulation of olfactory⁵ sensitivities in the malarial⁶ mosquito. It is thought that OBPs in the insect antennae⁷ and mouth parts function to concentrate odorant molecules⁸ and assist in their transport to the actual olfactory receptors, thereby⁹ allowing for odorant detection. The team revealed daily rhythmic¹⁰ protein abundance of OBPs, having higher concentrations in the mosquito's sensory organs at night than during the day. This discovery could change the way we look at protecting ourselves from these disease-carrying pests.

 

The team also included Matthew M. Champion, Eck Institute for Global Health Research Assistant Professor in the Department of Chemistry and Biochemistry, who specializes in proteomics.

 

This study utilized¹¹ mass spectrometry to quantify protein abundance in mosquito sensory organs, and electroantennograms to determine the response induced by host odorants at different times of the day. The coincident times of peak protein abundance, olfactory sensitivity and biting behavior reflect the extraordinarily¹² fine-tuned control of mosquito physiology¹³. Olfactory protein abundance and olfactory sensitivity are high when needed (at night) and low when not required (daytime).

 

Samuel Rund, a doctoral candidate in the laboratory of Duffield and a former Eck Institute for Global Health Fellow, and Nicolle Bonar, a visiting undergraduate student from Queens University of Ontario, Canada, were the lead authors on this research. The Notre Dame team also included then-undergraduate student John Ghazi, Class of 2012; undergraduate Cameron Houk, Class of '14; and graduate student Matthew Leming.

 

Rund noted¹⁴, "This was an exciting opportunity to bring many people and techniques together to make some really fascinating findings on the mosquito's ability to smell humans, its host. Just think, during the day the mosquito is sleeping and doesn't need to smell you. But when the sun goes down, the mosquito's olfactory system becomes extra-sensitive, and she is ready to smell and then bite you."

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