With the help of beneficial bacteria, plants can slam the door when disease pathogens come knocking, University of Delaware researchers have discovered. A scientific team under the leadership of Harsh Bais, assistant professor of plant and soil sciences in UD's College of Agriculture and Natural Resources, found that when pathogens attempt to invade a plant through the tiny open pores in its leaves, a surprising ally comes to the rescue. Soil bacteria at the plant's roots signal the leaf pores to close, thwarting¹（妨碍） infection.

 

The fascinating defense² response is documented in video and micrographs of live plants taken by confocal（共焦的） and scanning electron microscopes at UD's Bio-Imaging Center at the Delaware Biotechnology Institute.

 

The research, which explored the interaction between the soil bacterium³ Bacillus subtilis and the laboratory plant Arabidopsis thaliana, is published in the August issue of The Plant Journal. The findings underscore both the importance of root-based processes in plant defense and the potential for bolstering⁴ plant immunity⁵ naturally through the emerging field of probiotics（益生菌）.

 

Postdoctoral researcher Amutha Sampath Kumar is the lead author of the journal article. In addition to Bais, the co-authors include postdoctoral researcher Venkatachalam Lakshmanan, researchers Jeffrey L. Caplan, Deborah Powell and Kirk J. Czymmek of UD's Bio-Imaging Center, and Delphis F. Levia, associate professor of geography. The National Science Foundation, University of Delaware Research Foundation and Delaware Experimental Program to Stimulate⁶ Competitive Research (EPSCoR) provided funding for the study.

 

Millions of stomata, consisting of microscopic⁷ pores surrounded by guard cells, cover the above-ground parts of plants, from the stems to the flower petals⁸. The pores resemble tiny mouths, or doors, which the guard cells open and close to allow carbon dioxide, oxygen, water and minerals in and out of the plant.

 

Pathogens also can slip through these stomata（气孔） and begin infecting the plant. However, as Bais's team confirmed, this invasion is halted when the beneficial bacterium Bacillus subtilis is present in the soil where the plant is rooted. The finding was based on tests of approximately 3,000 Arabidopsis plants inoculated⁹ with the foliar pathogen Pseudomonas syringae pathovar tomato DC3000 (PstDC3000) during a year-long period.

 

When a foliar pathogen attacks, as shown in previous research by Bais and his group, the plant recruits Bacillus subtilis to help and facilitates its multiplication¹⁰. The Bacillus subtilis bacteria bind¹¹ to the plant's roots and invoke¹² abscisic acid and salicylic acid signaling pathways to close the stomata.

 

Abscisic acid and salicylic acid are both important hormones¹³ involved in plant defense. When a plant encounters adverse¹⁴ environmental conditions, such as drought, for example, abscisic acid triggers the stomata to shut tightly to prevent the plant from dehydrating.

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