Blood clots¹ play an unexpected role in protecting the body from the deadly effects of bacteria by absorbing bacterial³ toxins⁴, researchers at the University of California, Davis, have found. The research was published Dec. 2 in the journal PLoS ONE. "It's a significant addition to the short list of defenses that animals use to protect themselves against toxin⁵-induced sepsis," said Peter Armstrong, professor of molecular⁶ and cellular⁷ biology at UC Davis and senior author on the paper.

 

Even with modern antibiotics⁸, septic shock from bacterial infections afflicts⁹ about 300,000 people a year in the U.S., with a mortality rate of 30 to 50 percent. Septic shock is caused by Gram-negative bacteria, which release a toxin called lipopolysaccharide（脂多糖） or endotoxin（内毒素）. In small amounts, lipopolysaccharide triggers inflammation. When infections with these bacteria get out of hand, lipopolysaccharide courses through the bloodstream, causing catastrophic damage to organs and tissues.

 

These toxins cause disease in a variety of animal species -- lipopolysaccharide is also toxic¹⁰ to both horseshoe crabs¹¹ and lobsters¹², separated from humans by hundreds of millions of years of evolution. In humans and other mammals, blood clots quickly form from a mix of specialized¹³ blood cells and protein fibers¹⁴. Arthropods like horseshoe crabs and lobsters can also form clots in response to injury, with a different mix of cells and proteins.

 

Clots protect and help to seal wounds, prevent blood or body fluids from leaking out and form a physical barrier that entangles¹⁵ and blocks bacteria from entering the body. The new study shows that they also actively¹⁶ soak up lipopolysaccharide, reducing its release from the wound site into the body, where it could cause disease or even death.

 

Armstrong's laboratory had previously¹⁷ developed fluorescent¹⁸ labels to show that a lipopolysaccharide-like molecule¹⁹ is present in chloroplasts, structures inside cells of green plants that carry out photosynthesis²⁰（光合作用） and are thought to be descended²¹ from bacteria. As he also studies the role of blood clots in resisting infections, Armstrong decided²² to test the same techniques on blood clots that had been exposed to bacteria or to bacterial lipopolysaccharide. The fluorescent probes lit up the clots, showing that the clot² fibers bound lipopolysaccharide to their surfaces.

 

"I was ecstatic," Armstrong said. "It was one of those moments that makes the rest of the slogging worthwhile."

 

Armstrong and colleagues Margaret Armstrong at UC Davis and Frederick Rickles at George Washington University looked at clots of blood, or its equivalent, from humans, mice, lobsters and horseshoe crabs. In all four species, they found that fluorescently tagged lipopolysaccharide was bound to the fibers of the blood clot. The toxin was too tightly attached to be readily removed by chemical treatments that remove weakly bound macromolecules from proteins.

 

During a sabbatical leave in the laboratory of Dr. Bruce Furie at Beth Deaconess Medical Center and Harvard University, Armstrong was also able to film clots in blood vessels²³ of live mice and showed that these in vivo clots took up lipopolysaccharide in real time. These in vivo experiments, he said, confirm the bench-top observations and offer new insights into the pathology（病理学） of sepsis（败血症）.

 

One of the deadly consequences of septic shock is disseminated²⁴ intravascular coagulation²⁵, when blood clots form rapidly throughout the body. But the new results suggest that on a small and local scale, this might be part of a protective mechanism²⁶ against sepsis -- these intravascular clots can soak up quantities of lipopolysaccharide from the blood. They also show that rather than being a simple physical barrier, blood clots play an active and dynamic role in protecting the body from infections.

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