丝蛋白可在高温下保存血样
文章来源:未知 文章作者:enread 发布时间:2016-05-11 08:34 字体: [ ]  进入论坛
(单词翻译:双击或拖选)
Researchers at Tufts University have stabilized1 blood samples for long periods of time without refrigeration and at high temperatures by encapsulating them in air-dried silk protein. The technique, which is published online this week in the Proceedings2 of the National Academy of Sciences, has broad applications for clinical care and research that rely on accurate analysis of blood and other biofluids. Blood contains proteins, enzymes3, lipids, metabolites, and peptides that serve as biomarkers for health screening, monitoring and diagnostics. Both research and clinical care often require blood to be collected outside a laboratory. However, unless stored at controlled temperatures, these biomarkers rapidly deteriorate4, jeopardizing5 the accuracy of subsequent laboratory analysis. Existing alternative collection and storage solutions, such as drying blood on paper cards, still fail to effectively protect biomarkers from heat and humidity.
 
The Tufts scientists successfully mixed a solution or a powder of purified silk fibroin protein extracted from silkworm cocoons6 with blood or plasma7 and air-dried the mixture. The air-dried silk films were stored at temperatures between 22 and 45 degrees C (71.6 to 113 degrees F). At set intervals8, encapsulated blood samples were recovered by dissolving the films in water and analyzed9.
 
"This approach should facilitate outpatient blood collection for disease screening and monitoring, particularly for underserved populations, and also serve needs of researchers and clinicians without access to centralized testing facilities. For example, this could support large-scale epidemiologic studies or remote pharmacological trials," said senior and corresponding author David L. Kaplan, Ph.D., Stern Family Professor in the Department of Biomedical Engineering at Tufts School of Engineering. 
 
 "We found that biomarkers could be successfully analyzed even after storage for 84 days at temperatures up to 113 degrees F. Encapsulation of samples in silk provided better protection than the traditional approach of drying on paper, especially at these elevated temperatures which a shipment might encounter during overseas or summer transport," said the paper's co-first author Jonathan A. Kluge, who earned both his Ph.D. and B.S. from Tufts School of Engineering and was a postdoctoral associate in the Kaplan lab when the research was done. 
 
The paper noted10 that the silk-based technique requires accurate starting volumes of the blood or other specimens11 to be known, and salts or other buffers12 are needed to reconstitute samples for accurate testing of certain markers.


点击收听单词发音收听单词发音  

1 stabilized 02f3efdac3635abcf70576f3b5d20e56     
v.(使)稳定, (使)稳固( stabilize的过去式和过去分词 )
参考例句:
  • The patient's condition stabilized. 患者的病情稳定下来。
  • His blood pressure has stabilized. 他的血压已经稳定下来了。 来自《现代英汉综合大词典》
2 proceedings Wk2zvX     
n.进程,过程,议程;诉讼(程序);公报
参考例句:
  • He was released on bail pending committal proceedings. 他交保获释正在候审。
  • to initiate legal proceedings against sb 对某人提起诉讼
3 enzymes 7881ad8ce9c83424f7874e70266ed2d8     
n. 酶,酵素
参考例句:
  • It was said that washing powders containing enzymes remove stains more efficiently. 据说加酶洗衣粉除污更有效。
  • Among the enzymes which are particularly effective are pepsin, papain. 在酶当中特别有效的是胃朊酶、木瓜酶。
4 deteriorate Zm8zW     
v.变坏;恶化;退化
参考例句:
  • Do you think relations between China and Japan will continue to deteriorate?你认为中日关系会继续恶化吗?
  • He held that this would only cause the situation to deteriorate further.他认为,这只会使局势更加恶化。
5 jeopardizing 6ec88fcb3085928bbf8588a5c3ba3e65     
危及,损害( jeopardize的现在分词 )
参考例句:
  • Plans may also become inefficient in the attainment of objectives by jeopardizing group satisfactions. 用危及群体利益方法去达到目标的计划,也是无效率的。
  • That boosted government revenues in the short term, but is now jeopardizing them. Morales将天然气工业,电信业和部分采矿业收归国有的举措吓跑了投资者们。
6 cocoons 5dceb05da0afff0d0dbbf29f10373b59     
n.茧,蚕茧( cocoon的名词复数 )v.茧,蚕茧( cocoon的第三人称单数 )
参考例句:
  • The silkworms have gone into the bushes to spin their cocoons. 蚕上山了。 来自《现代汉英综合大词典》
  • In two more days the " little darlings" would spin their cocoons. 再得两天,“宝宝”可以上山。 来自汉英文学 - 春蚕
7 plasma z2xzC     
n.血浆,细胞质,乳清
参考例句:
  • Keep some blood plasma back for the serious cases.留一些血浆给重病号。
  • The plasma is the liquid portion of blood that is free of cells .血浆是血液的液体部分,不包含各种细胞。
8 intervals f46c9d8b430e8c86dea610ec56b7cbef     
n.[军事]间隔( interval的名词复数 );间隔时间;[数学]区间;(戏剧、电影或音乐会的)幕间休息
参考例句:
  • The forecast said there would be sunny intervals and showers. 预报间晴,有阵雨。
  • Meetings take place at fortnightly intervals. 每两周开一次会。
9 analyzed 483f1acae53789fbee273a644fdcda80     
v.分析( analyze的过去式和过去分词 );分解;解释;对…进行心理分析
参考例句:
  • The doctors analyzed the blood sample for anemia. 医生们分析了贫血的血样。 来自《简明英汉词典》
  • The young man did not analyze the process of his captivation and enrapturement, for love to him was a mystery and could not be analyzed. 这年轻人没有分析自己蛊惑著迷的过程,因为对他来说,爱是个不可分析的迷。 来自《简明英汉词典》
10 noted 5n4zXc     
adj.著名的,知名的
参考例句:
  • The local hotel is noted for its good table.当地的那家酒店以餐食精美而著称。
  • Jim is noted for arriving late for work.吉姆上班迟到出了名。
11 specimens 91fc365099a256001af897127174fcce     
n.样品( specimen的名词复数 );范例;(化验的)抽样;某种类型的人
参考例句:
  • Astronauts have brought back specimens of rock from the moon. 宇航员从月球带回了岩石标本。
  • The traveler brought back some specimens of the rocks from the mountains. 那位旅行者从山上带回了一些岩石标本。 来自《简明英汉词典》
12 buffers 4d293ef273d93a5411725a8223efc83e     
起缓冲作用的人(或物)( buffer的名词复数 ); 缓冲器; 减震器; 愚蠢老头
参考例句:
  • To allocate and schedule the use of buffers. 分配和计划缓冲器的使用。
  • Number of times the stream has paused due to insufficient stream buffers. 由于流缓冲区不足导致流程暂停的次数。
TAG标签: blood protein silk
发表评论
请自觉遵守互联网相关的政策法规,严禁发布色情、暴力、反动的言论。
评价:
表情:
验证码:点击我更换图片